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Image Search Results
Journal: Frontiers in Pharmacology
Article Title: Dopamine Homeostasis Imbalance and Dopamine Receptors-Mediated AC/cAMP/PKA Pathway Activation are Involved in Aconitine-Induced Neurological Impairment in Zebrafish and SH-SY5Y Cells
doi: 10.3389/fphar.2022.837810
Figure Lengend Snippet: Cell damage of zebrafish larvaes and SH-SY5Y cells caused by aconitine. (A) The images of aconitine-treated larvaes by DCFH-DA (100×), n = 6. (B) The images of aconitine-exposed larvaes by TUNEL assay (100×), n = 3. (C) Cell viability of SH-SY5Y cells after 24 h or 48 h aconitine exposure, n = 8. (D) The images of aconitine-treated SH-SY5Y cells by DCFH-DA (200×), n = 3. (E) SH-SY5Y cell apoptosis aconitine-induced based on Annexin V/PI double staining by flow cytometry, n = 3. All photographs were taken under a stereoscopic fluorescence microscope or confocal microscope. The values are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control groups.
Article Snippet: After staining with 25 μM of working solution (freshly prepared) at 28°C for 1 h in the dark, zebrafish larvaes were washed with fresh fish water three times and were taken photos under a
Techniques: TUNEL Assay, Double Staining, Flow Cytometry, Fluorescence, Microscopy, Control
Journal: Frontiers in Pharmacology
Article Title: Dopamine Homeostasis Imbalance and Dopamine Receptors-Mediated AC/cAMP/PKA Pathway Activation are Involved in Aconitine-Induced Neurological Impairment in Zebrafish and SH-SY5Y Cells
doi: 10.3389/fphar.2022.837810
Figure Lengend Snippet: Effects of aconitine on TH, MAO, DAT and VMAT2 expressions in zebrafish larvaes and SH-SY5Y cells. (A) The mRNA expressions of th , mao , dat , vmat2 with aconitine exposure in zebrafish larvaes, n = 6. (B) TH and VMAT2 positive cells were investigated using immunostaining and Tg (vmat2:GFP) transgenic zebrafish larvaes, respectively, n = 5. (C) Effect of different exposure times of 1 μM aconitine on the protein expression of TH, n = 4. (D) The protein levels of TH, MAO-B, DAT and VMAT2 with aconitine exposure in zebrafish larvaes, n = 4. (E) The mRNA expressions of th , mao-b , dat , vmat2 with aconitine exposure in SH-SY5Y cells, n = 3. (F) The protein levels of TH, MAO-B, DAT, and VMAT2 with aconitine exposure in SH-SY5Y cells, n = 7. All photographs in which the green fluorescence represented the immune-reactive cells were taken under a confocal microscope (100×). The values are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control groups.
Article Snippet: After staining with 25 μM of working solution (freshly prepared) at 28°C for 1 h in the dark, zebrafish larvaes were washed with fresh fish water three times and were taken photos under a
Techniques: Immunostaining, Transgenic Assay, Expressing, Fluorescence, Microscopy, Control